Table 4.
Flow cytometry analysis of monocyte subsets and P-selectin expression following nitrate-depleted (placebo) and nitrate-rich beetroot juice ingestion.
| Variable | LO-NI | HI-NI | 2-Way ANOVA | ||||||
|---|---|---|---|---|---|---|---|---|---|
| PRE | 3 h POST | 6 h POST | PRE | 3 h POST | 6 h POST | Time | Treatment | Time × Treatment | |
| Classical CD14++ CD16− monocytes | 90.0 ± 4.1 | 91.6 ± 2.8 | 91.2 ± 3.7 | 91.1 ± 3.6 | 92.1 ± 3.2 | 89.7 ± 2.2 | 0.047 | 0.991 | 0.112 |
| Intermediate CD14++ CD16+ monocytes | 4.3 ± 1.5 | 3.9 ± 1.5 | 3.3 ± 1.6 * | 4.0 ± 1.5 | 3.5 ± 1.3 | 4.9 ± 1.0 * | 0.183 | 0.532 | 0.001 |
| Non-classical CD14+ CD16++ monocytes | 5.4 ± 2.7 | 4.4 ± 1.4 | 5.0 ± 2.4 | 4.2 ± 2.0 | 4.4 ± 2.1 | 5.4 ± 1.8 | 0.235 | 0.722 | 0.239 |
| P-selectin (CD42a) | 3083 ± 1657 | 3731 ± 1682 | 3104 ± 1202 | 3908 ± 1404 | 3810 ± 2073 | 3575 ± 1937 | 0.385 | 0.442 | 0.495 |
Data indicated as mean values ± standard deviation (SD), n = 12. * Significantly different from PRE values, p < 0.05; LO-NI, low-nitrate beetroot juice; HI-NI, high-nitrate beetroot juice; PRE, pre-ingestion (baseline); 3 h POST, 3 h post-ingestion; 6 h POST, 6 h post-ingestion.