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. 2017 Nov 2;14(13):1389–1401. doi: 10.7150/ijms.21894

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Expression of osteoblast-specific genes and RANKL during osteoblastic differentiation of hPDCs. Values are mean ±SEM (n=3). A and B: Expression of collagen type I and Runx2 was significantly increased in the hPDCs at day 10 of culture, but was markedly decreased at the 21-day time point. Conversely, expression of osteocalcin, osteopontin, and RANKL mRNA increased in a time-dependent manner up to day 21 of culture. C: Levels of RANKL protein also increased over the 3-week experimental duration and were highest in osteogenic-conditioned media collected from hPDCs at day 21 of culture.

Expression of osteoblast-specific genes and RANKL during osteoblastic differentiation of hPDCs. Values are mean ±SEM (n=3). A and B: Expression of collagen type I and Runx2 was significantly increased in the hPDCs at day 10 of culture, but was markedly decreased at the 21-day time point. Conversely, expression of osteocalcin, osteopontin, and RANKL mRNA increased in a time-dependent manner up to day 21 of culture. C: Levels of RANKL protein also increased over the 3-week experimental duration and were highest in osteogenic-conditioned media collected from hPDCs at day 21 of culture.