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. 2017 Nov 2;14(13):1389–1401. doi: 10.7150/ijms.21894

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Expression of osteoclastogenic markers and osteoclast number. hOBs were co-cultured with CD34+ HSCs and osteogenic-conditioned medium from hPDCs undergoing osteoblastic differentiation (collected 48 h after 0, 10, and 21 days of culture). A: Expression of osteoclastogenesis markers, TRAP, cathepsin K, and integrin beta-3, was the markedly higher in α-MEM + day 21-osteogenic CM group. B: Osteoclast number, indicated by TRAP staining, was significantly increased in the α-MEM + day 21-osteogenic CM group. Scale bar = 250 µm.

Expression of osteoclastogenic markers and osteoclast number. hOBs were co-cultured with CD34+ HSCs and osteogenic-conditioned medium from hPDCs undergoing osteoblastic differentiation (collected 48 h after 0, 10, and 21 days of culture). A: Expression of osteoclastogenesis markers, TRAP, cathepsin K, and integrin beta-3, was the markedly higher in α-MEM + day 21-osteogenic CM group. B: Osteoclast number, indicated by TRAP staining, was significantly increased in the α-MEM + day 21-osteogenic CM group. Scale bar = 250 µm.