Table 3.
Primers used in qPCR
| Target gene | Sequence | Product size (bp) | Annealing temperature | |
|---|---|---|---|---|
| 1 | Collagen Type I | F: TGAATACAAAACCACCAAGACC R: GAGTTTACAGGAAGCAGACATG |
111 | 60℃ |
| 2 | Runx2 | F: CAAATCCTCCCCAAGTAGCT R: ATACTGGGATGAGGAATGCG |
117 | 60℃ |
| 3 | Osteopontin | F: TTGCAGCTTCTCAGCCAA R: GGAGGCAAAAGCAAATCACCT |
132 | 60℃ |
| 4 | Osteocalcin | F: TCACACTCCTCGCCCTATTG R: ACTTTTGCTGGACTCTGCAC |
89 | 60℃ |
| 5 | RANKL | F: GGCAGCACGCTATTAAATCC R: GTCGCCAAACAGATTCATCC |
124 | 60℃ |
| 6 | TRAP | F: GATCCTGGGTGCAGACTTCA R: GCGCTTGGAGATCTTAGAGT |
211 | 60℃ |
| 7 | Cathepsin K | F: ACCGGGGTATTGACTCTGAA R: GAGTCAGGCTTGCATCAAT |
189 | 60℃ |
| 8 | Integrin beta 3 | F: TCGAGTTCCCAGTGAGTGAG R: GACAGGTCCATCAAGTAGTAG |
202 | 60℃ |