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. 2017 Nov 29;17:113. doi: 10.1186/s12935-017-0485-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Overexpression of CMTM5 inhibits HCC cell growth and metastasis in vitro. a Lentivirus vector pLenti6.3-CMTM5-IRES-EGFP and the mock control lentivirus were transfected into Huh7 cells. Transfection efficiency was confirmed by qRT-PCR and western blotting. b Cell growth was assessed by time course CCK-8 assay. c Cell apoptosis was determined by flow cytometry using the Annexin V/PI staining. d Cell metastatic and invasion was assessed by Transwell assay. Data are shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, compared with Huh7-Mock group (One-Way ANOVA with Dunnett’s test)