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. 2016 Sep 15;1:16018. doi: 10.1038/npjvaccines.2016.18

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Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Intestinal content of a dog vaccinated with VSPs purified from DAS-altered trophozoites contains antibodies against VSPs of G. lamblia assemblage B and G. muris. Phase-contrast (a,c) and immunofluorescence microscopy (b,d) of G. lamblia clone GS/M-H7 from assemblage B (a,b) and G. muris obtained from naturally infected experimental rats (c,d) confronted with intestinal content from a dog immunised with the entire repertoire of VSPs (DAS). An anti-dog IgA (red) was used for immunofluorescence microscopy. White bar represents 100 μm.