Table 2. Immunoglobulin subtypes anti-Giardia VSPs in infected puppies and kittens.
| Animal/infection | Serum Ig | Intestinal Ig | Animal/infection | Serum Ig | Intestinal Ig |
|---|---|---|---|---|---|
| Puppy 3/PBS | None | None | Kitten 3/PBS | None | None |
| Puppy 6/WB9B10 | A, G, M | A | Kitten 6/WB9B10 | A, G, M | A |
| Puppy 9/DAS | A, G, M | A | Kitten 9/DAS | A, G, M | A |
| Puppy 12/RAS | A, G, M | A | Kitten 12/RAS | A, G, M | A |
Sera or intestinal contents of infected or control animals were used in agglutination assays in 96-well plates. Approximately 5×105 trophozoites were incubated at 4 °C for 1 h with heat-inactivated intestinal secretions and sera in TYI-S-33 medium without bovine serum. The well content was mixed to assess agglutination of trophozoites by microscopy and to reveal antibodies using different anti-dog or anti-cat immunoglobulins.