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. 2017 Nov 30;10:230. doi: 10.1186/s13068-017-0918-6

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Transformation vector map and relative transcript abundance of TcEG1 in transgenic switchgrass. a pANIC-10A-TcEG1 vector used for expression of TcEG1 in transgenic switchgrass. LB: left border; PvUbi2: switchgrass ubiquitin 2 promoter and intron; hph: hygromycin B phosphotransferase coding region; 35S T: 35S terminator sequence; PvUbi1: switchgrass ubiquitin 1 promoter and intron; pporRFP: Porites porites orange fluorescent protein coding region; NOS T: Agrobacterium tumefaciens nos terminator sequence; ZmUbi1: maize ubiquitin 1 promoter; R1 and R2: attR1 and attR2 recombinase sites 1 and 2; TcEG1: TcEG1 cDNA open reading frame; AcV5: epitope tag; RB: right border; Kan^r: kanamycin resistance gene; ColE1: origin of replication in E. coli; pVS1: origin of replication in A. tumefaciens; OCS T: octopine synthase terminator sequence. b Relative transcript abundance of TcEG1 in stem internodes from transgenic events (Tc-1 to Tc-12). Relative expression analysis was determined by qRT-PCR and normalized to switchgrass ubiquitin 1 (PvUbi1). Bars represent mean values of three replicates ± standard error. Bars represented by different letters are significantly different as calculated by LSD (p ≤ 0.05)