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. 2017 Nov 30;10:230. doi: 10.1186/s13068-017-0918-6

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Endoglucanase activity (units/mg of protein) from fresh leaves of transgenic TcEG1 plants. a Endoglucanase activity measurement using carboxymethyl cellulose (CMC) as substrate at pH 12.0 on TcEG1 extracted from fresh leaves. Bars represent mean values of three replicates ± standard error for each transgenic event. Bars represented by different letters are significantly different as calculated by LSD (p ≤ 0.05). b Gradient pH measurement of endoglucanase activity of TcEG1 extracted from fresh leaves of transgenic event Tc-1. Data points represent mean values of three replicates ± standard error. Data points represented by different letters are significantly different as calculated by LSD (p ≤ 0.05)