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. 2017 Nov 29;19:128. doi: 10.1186/s13058-017-0921-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Obesity enhances luminal and decreases basal/myoepithelial cell populations. Mammary epithelial cells isolated from control (Con) and obese (high-fat diet (HFD)) C57Bl/6 mice were stained with EpCAM and CD49f and quantified using flow cytometry (a, b). a Representative contour plots depicting gates for luminal cells (EpCAM^hiCD49f^lo) and basal cells (EpCAM^loCD49f^hi). The percentage of luminal and basal cells were quantified from total mammary-lineage-positive cells in three experiments (n = 9 mice/group). b Results were determined as a ratio of luminal to basal cells from obese mice compared with control mice and represented as mean ± s.d. Human breast epithelial cells were isolated from reduction mammoplasty samples, stained with EpCAM and CD49f, and quantified using flow cytometry (n = 11 patient samples). c Representative contour plot depicting gates for mature luminal (ML, EpCAM^hiCD49f^-), luminal progenitor (LP, EpCAM^hiCD49f⁺), mature basal (MB, EpCAM^loCD49f⁺), and basal progenitor cells (BP, EpCAM^-CD49f⁺). d Correlation scatter plot with best fit line representing the ratio of the percentage of luminal to basal/myoepithelial cells. The percentage of positive cells was the number of positive cells in the gate compared to the total number of mammary-lineage-positive cells. Correlation scatter plot with best fit line representing the percentage of MB (e), BP (f), ML (g), and LP (h) against body mass index (BMI)