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. 2016 Aug 25;1:16010. doi: 10.1038/npjvaccines.2016.10

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Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Ab-producing cells induced by ID immunisation with gp140+Poly(I:C). IgG (a) and IgA (b) SFC were measured by ELISPOT assay 5 days after the last ID immunisation with gp140+Poly(I:C) in uterus, colon, small intestine and bone marrow (left panels) and in cLN, mLN, gLN and spleen (right panels). Data are expressed as mean+s.e.m. of gp140-specific SFC/10⁶ total (right panels) or CD45⁺ cells (left panels) pooled from 6–8 mice /group. Statsitical analyses were performed using one-way analysis of variance (ANOVA) with Tukey’s multiple comparison test.