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. 2017 Nov 30;10:266. doi: 10.1186/s13068-017-0954-2

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Experimental design. Switchgrass cell suspension cultures were transferred to fresh medium containing 9 μM 2,4-D (controls, blue), or to medium with 0.9 μM 2,4-D supplemented with 0.2 μM BL (induced, orange), and harvested at the times shown. The changes in cell wall composition of induced and non-induced samples were analyzed by biochemical and immunochemical approaches at all the times shown. Microarray analysis was performed on induced samples harvested at 0, 6 h, 1, 3, and 7 days after transfer, and on non-induced samples 1 and 7 days after transfer. Comparative transcriptomics and correlation with changes in cell wall composition were applied to identify putative cell wall-related genes