Fig. 2.

Glucan plus xylan releases from hydrolysis of solids produced by CELF pretreatment of (a) corn stover and (b) Populus and DA pretreatment of (c) corn stover and (d) Populus by fungal enzymes at 50 °C at loadings of 2, 5, and 15 mg of total enzyme protein/g glucan in biomass before deconstruction and by C. thermocellum (2% v/v inoculum) at 60 °C. Because C. thermocellum fermentation of CELF-pretreated solids was complete in 48 h, its 120- and 168-h time points are extensions of 48-h sugar release. All anaerobic digestion and enzymatic hydrolysis experiments were run in duplicate with mean values shown. Error bars in the graph are one standard deviation. The sugar release for enzymatic hydrolysis refers to sugars recovered in the solution as determined by direct measurement. Sugar release for CBP refers to the amount of sugars solubilized as determined by analysis of the carbohydrates in the residual solids