Table 2.

PCR primers and amplification conditions used to analysis of cdt genes for Campylobacter isolates.

Genus	Gene	Sequence (5’→3’)	Amplification (1) Condition	Size (bp)
Campylobacter jejuni	cdtA	F: AGGACTTGAACCTACTTTTC	94 °C, 30 s −55 °C, 30 s −72 °C, 30 s	631
		R: AGGTGGAGTAGTTAAAAACC
	cdtB	F: ATCTTTTAACCTTGCTTTTGC	94 °C, 30 s −56 °C, 30 s −72 °C, 30 s	714
		R: GCAAGCATTAAAATCGCAGC
	cdtC	F: TTTAGCCTTTGCAACTCCTA	94 °C, 30 s −55 °C, 30 s −72 °C, 30 s	524
		R: AAGGGGTAGCAGCTGTTAA
Campylobacter coli	cdtA	F: ATTGCCAAGGCTAAAATCTC	94 °C, 30 s −55 °C, 30 s −72 °C, 30 s	329
		R: GATAAAGTCTCCAAAACTGC
	cdtB	F: TTTAATGTATTATTTGCCGC	94 °C, 30 s −56 °C, 30 s −72 °C, 30 s	413
		R: TCATTGCCTATGCGTATG
	cdtC	F: TAGGGATATGCACGCAAAAG	94 °C, 30 s −55 °C, 30 s −72 °C, 30 s	313
		R: GCTTAATACAGTTACGATAG

⁽¹⁾ Amplification: denaturation-annealing-extension.