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. 2017 Nov 29;18:529. doi: 10.1186/s12859-017-1934-z

Fig. 7.

Fig. 7

A mixed-world ImageJ 1.x + ImageJ2 script. This example Python script (panel d) uses ImageJ Ops to preprocess a confocal image and perform an automatic thresholding (panel b). ImageJ 1.x’s Analyze Particles routine is then called to isolate (panel a) and measure (panel c) foreground objects. Script contributed by Brian Northan, True North Intelligent Algorithms LLC. This script is available within ImageJ as a sample from the Tutorials submenu of the Script Editor’s Templates menu