Figure 2. The effect of tungstate on H3K9me2 and H3K4me3 in BEAS-2B and A549 cells.

BEAS-2B cells were exposed to 0, 1, 2.5 and 5 mM of Na₂WO₄ for 48-hours, and then cells were allowed to proliferate in the absence of tungstate for 48 hours, for a washout. Histones were extracted after treatment and washout.

A, H3K9me2 was detected using Western blotting as described in Materials and Methods. The same membrane was stripped and reprobed with H3 antibody as loading control.

B, H3K4me3 was detected using Western blotting. The same membrane was stripped and reprobed using H3 antibody as loading control.

C, A549 cells were exposed to 0, 2.5 and 5 mM of Na₂WO₄ for 48-hours, and then cells were allowed to proliferate in the absence of tungsten for 48 hours, for a washout. Histones were extracted after treatment and washout. H3K4me3 was detected using Western and the same membrane was stripped and reprobed using H3 antibody as loading control.

The results were repeated in three independent experiments; one representative blot is shown here. Graphical representations of relative intensities are calculated by ImageJ.

Statistical significance was calculated using an unpaired, two-tailed t test

* p-value < 0.05

** p-value < 0.01

*** p-value < 0.001

Error bars represent Standard Deviation.