Figure 5. The effect of methionine depletion on JMJD1A and JARID1A protein levels in BEAS-2B cells treated with tungstate.

BEAS-2B cells were seeded with DMEM complete medium. The following day, cells were pre-incubated for 4 hours in methionine-deficient DMEM, and then cells were exposed to either 0, 1, 2.5 or 5 mM Na₂WO₄ for 24-hours. Nuclear extracts were extracted after treatment.

A, JMJD1A was detected using Western blotting. The same membrane was reprobed with Lamin A antibody as loading control.

B, JARID1A was detected using Western blotting. The same membrane was reprobed using Lamin A antibody as loading control

The results were repeated in three independent experiments; one representative blot is shown here. The intensity of the bands was quantified using ImageJ, and values were normalized to the each loading control and plotted in the graph. Error bars represent SD.

Statistical significance was calculated using an unpaired, two-tailed t test

* p-value < 0.05

** p-value < 0.01

*** p-value < 0.001