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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Nat Microbiol. 2017 Aug 14;2(10):1403–1414. doi: 10.1038/s41564-017-0007-4

Figure 4. GNF-Pf-5660 causes a decrease in Hz and total heme iron levels in wild-type ABS Dd2-B2 and mutant HHQ-resistant PfMDR1selY290F parasites, the latter at significantly higher compound concentrations.

Figure 4

Synchronized parasites were exposed to GNF-Pf-5660 or the reference antimalarials MQ and LMF and fractionated in order to measure Hb, free heme, or Hz iron. Total heme iron represents the sum of these three values. Results show Hb, free heme (which could be loosely associated with lipids or other biomolecules), Hz and total heme iron levels (as fg/cell) in parasites exposed to a range of IC50 values (0.1, 0.25, 0.5, 1.0 and 2.0′). Mean ± SEM amounts were calculated from two independent experiments and statistical significance was assessed by a two-tailed t-test. *P<0.05, **P<0.01, ***P<0.001. All data are available in Supplementary Table 15.