Table 14.1.

Release of glycans from glycoproteins is attained through different methods.

N-glycan	Chemical Release
	Using anhydrous hydrazine at 95 °C	(Takasaki, Mizuochi, & Kobata, 1982)
	Oxidation using sodium hypochlorite	(Song, Ju, Lasanajak, Kudelka, Smith, & Cummings, 2016)
	Enzymatic Release
	PNGase F purified from Flavobacterium meningosepticum	(Takahashi et al., 1978; Tarentino, Gomez, & Plummer, 1985)
	PNGase A purified from almond Prunus amygdalus var. dulcis	(Takahashi et al., 1978; Tretter et al., 1991)
	Endoglycosidase F1 purified from Elizabethkingia miricola	(Trimble et al., 1991)
	Endoglycosidase F2 purified from Elizabethkingia miricola	(Trimble et al., 1991)
	Endoglycosidase F3 purified from Elizabethkingia miricola	(Trimble et al., 1991)
	Endoglycosidase H purified from Streptomyces plicatus	(Tarentino & Maley, 1974; Tarentino, Plummer, & Maley, 1974)
	Endo-β-N-acetylglucosaminidase (Endo-M) purified from Mucor hiemalis	(Kadowaki, Yamamoto, Fujisaki, Izumi, Tochikura, & Yokoyama, 1990)
O-glycan	Chemical Release
	Using anhydrous hydrazine at 60 °C	(Patel, Bruce, Merry, Bigge, Wormald, Jaques et al., 1993)
	Alkaline-β-elimination	(Carlson, 1968)
	Non-reductive β-elimination	(Hulsmeier, Gehrig, Geyer, Sack, Gottstein, Deplazes et al., 2002)
	Oxidation using sodium hypochlorite	(Song et al., 2016)
	Enzymatic Release
	Endo-α-N-acetylgalactosaminidase	(Huang et al., 1972)
	Enzymatic/Chemical Release
	Pronase followed by solid phase permethylation	(Goetz, Novotny, & Mechref, 2009)