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. Author manuscript; available in PMC: 2018 Jan 16.
Published in final edited form as: Methods Enzymol. 2017 Jan 16;585:397–429. doi: 10.1016/bs.mie.2016.11.009

Figure 7.2. Boronic acid enrichment and electrostatic repulsion hydrophilic interaction chromatography enrichment.

Figure 7.2

A. A covalent bond between the stationary media and the glycan hydroxyl groups allows for clean separations. Reproduced with permission (Ongay et al., 2012). B. Silica beads functionalized with polyethyleneimine attract uncharged glycans (right) while repelling charged glycans (left). Reproduced with permission (L. H. Zacharias, A. ; Song, E.; Zhao, J.; Zhu, R.; Mirzaei, P.; Mechref, Y., 2016).