Figure 4. AD^Flox/− tumors display genetic signature of angiosarcomas.

(A) Volcano plot of the Log₁₀ of the P value versus the log ratio fold difference in mRNA expression in AD^Flox/− angiosarcomas (n = 7) versus normal quadriceps femoris skeletal muscle (n = 4). Genes with P < 0.01 and log ratio > 2 (red) or log ratio < −2 (green) and genes of interest are labeled. (B) Gene ontology analysis with significantly enriched pathways in genes upregulated in tissue from (A) with P value < 0.05 and > 4-fold increased expression in angiosarcoma (578 genes). (C) Volcano plot of the Log₁₀ of the P value versus the log ratio fold difference in mRNA expression in AD^Flox/− angiosarcomas (n = 7) versus normal WT aortas (n = 4). Genes with P < 0.01 and log ratio > 2 (red) or log ratio < −2 (green) and genes of interest are labeled. (D) Gene ontology analysis with significantly enriched pathways in genes upregulated in tissue from (C) P value < 0.05 and > 4-fold increased expression in angiosarcoma (248 genes). (E) Expression of Apln and Aplnr by qRT-PCR in AD^Flox/− angiosarcomas (n = 3), normal aorta (n = 3), and normal quadriceps (n = 3), P < 0.05 for all comparisons of angiosarcoma versus normal tissue. (F) Immunoblot analysis of lysates from AD^Flox/− angiosarcomas and control normal aortas. Antibodies used are shown to the left.