Median-joining network showing the genetic relationship between highly pathogenic avian influenza A viruses subtype H5N8 isolated from commercial poultry farms (red circles) and the most identical wild bird viruses (blue circles) found in the Netherlands, 2016. Predicted median vectors are shown in yellow. The length of the line represents the genetic distance, and the number of nucleotide changes is indicated. Wild bird virus isolates used for this analysis are numbered 1–10 (Technical Appendix 1 Table 2). GISAID EpiFlu database (https://www.gisaid.org) accession numbers are shown in Technical Appendix 1 Table 1. EW, Eurasian wigeon; Go, gray goose; Ma, mallard; TD,= tufted duck.