Table. RT-PCR primers used for generation of amplicons used for sequencing CCHFV full genome from strains isolated from patient samples in Spain, 2016*.

Primer pair	5′ → 3′ sequence	5′ → 3′ position relative to reference strain AB1-2009	Amplified segment, kb	Temperature, °C†
CRCON+1	RWAAYGGRCTTRTGGAYACYTTCAC	123–147	S, 1.4	50.5
CRCON1R-S	TCTCAAAGATAYCGTTGCCGCAC	1,651–1,673
CRCON1F-M	TCTCAAAGAAAATACTTGCGGC	1–22	M, 2.3	48
CRCON4R-M	GCATYTCAGCATCTATTGCATT	2,326–22,347
CRCONF4F-M	TTGTAGAYCAAAGACTRGGCAG	1,775–1,796	M, 2.1	48
CRCON6R-M	GCCYGCTTCAATCAAGCTACA	3,829-3,849
CRCON6F-M	TCAATTGAGGCACCATGGGG	3,280–3,299	M, 2.1	50
CRCON8R-M	TCTCAAAGATATAGTGGCGGCA	5,348–5,369
CRCON1F-L	TCTCAAAGATATCAATCCCCCC	1–22	L, 2.2	48
CRCON3R-L	AGTGTCGAAAATGTRCAAATCTC	2,202--2224
CRCON3F-L	CCTGAAAGTGACCTCACCCGC	1,562–1,582	L, 3.1	55
CRCON6R-L	TTGGCATGCTTGCAGGGCTTAG	4,656–4,677
CRCON7F-L	TGCTTGCAGGGCTTAGTAGGCT	4,661–4,682	L, 2.3	50.5
CRCON9R-L	TCATGCATGCAACCACTGAAAT	6,948-6,969
CCHF-L2F	GAAGAGCTATATGACATAAGGC	6,137–6,158	L, 1.6	48
CCHF-L1R	TTGGCACTATCTTTCATTTGAC	7,752–7,773
CRCON10F-L	GGTAGTTCAGATGATTACGCAAA	7,616–7,638	L, 1.4	48
CRCON10R-L	CCTGTTAATTGTTTGCCACAA	9,019–9,039
CRCON7200F	ATGCAACAGGTTCTGAAAAATG	7,199–7,220	L, 3.5	48
CRCON11R-L	ATGCTCCTAGTGATGCCATAATG	10,743–10,765
CRCON12F-L	TCTTTTGAAGGTGAAGCATCTTG	10,585–10,607	L, 1.6	48
CCHF-L2R	TCTCAAAGAAATCGTTCCCCCCAC	12,149–12,172
*F, forward; L, large segment; M, medium segment; R, reverse; RT-PCR, reverse transcription PCR; S, small segment. †Melting temperature used.