Table 2. Results of the experimental exposure of frogs to Elizabethkingia miricola isolate FL160902, China, 2016*.
| Route of
infection |
Concentration, CFU/mL |
No.
frogs
per trial |
Cumulative no. deaths, by days after exposure† | Mortality,
% |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 2 |
4 |
6 |
8 |
10 |
12 |
14 |
||||
| Intramuscular injection‡ | 105 | 10 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 10 |
| 106 | 10 | 0 | 0 | 1 | 1 | 5 | 5 | 5 | 50 | |
| 107 | 10 | 1 | 3 | 6 | 7 | 7 | 7 | 7 | 70 | |
|
|
SPSS§ |
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
| Immersion
inoculation¶ |
106 |
10 |
3 |
7 |
10 |
10 |
10 |
10 |
10 |
100 |
| Cohabitation
inoculation# |
NA |
10 |
0 |
0 |
1 |
3 |
3 |
3 |
3 |
30 |
| Control | NA | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
*NA, not applicable. †Deaths after 14 d were not included. ‡Injection volume 200 μL. §An equivalent volume injection of 0.70% stroke-physiologic saline solution. ¶Immersed for 30 min in E. miricola suspension. #Frogs in this trial cohabited with frogs previously infected with E. miricola.