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Homogenous O2 evolution coupled to an oxygenase‐catalyzed oxyfunctionalization reaction. Water is oxidized by the photosynthetic cyanobacterium Synechocystis sp. PCC6803, yielding O2 and activated reduction equivalents. The heterologously introduced alkane monooxygenase system AlkBGT captures both O2 and the reduction equivalents, and catalyzes the regiospecific oxyfunctionalization of nonanoic acid methyl ester (NAME) to ω‐hydroxynonanoic acid methyl ester (H‐NAME).
