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. 2017 Oct 11;18(22):2226–2230. doi: 10.1002/cbic.201700478

Table 1.

Peroxidase and oxidase activities of the fusion enzymes.[a]

Fusion k obs [s−1] Fusion k obs [s−1]
enzyme Peroxidase Oxidase enzyme Peroxidase Oxidase
P‐ChitO* 7.7 (6.6) 1.0 (6.523) P‐HMFO 7.1 (6.6) 9.0 (2121)
P‐EugO 5.0 (6.6) 2.5 (1222) P‐HotAldO 8.6 (6.6) 0.43 (1.920)

[a] The peroxidase activity was measured by using Reactive Blue 19 as a substrate at pH 4.0. The oxidase activities of P‐EugO and P‐HMFO towards vanillyl alcohol were measured at pH 7.5 and 8.0, respectively. The activity of P‐HotAldO towards xylitol was measured at pH 7.5, and the activity of P‐ChitO* towards cellobiose was measured at pH 7.6. The values in parentheses indicate the k cat. values of the separate enzymes, as determined for SviDyP (see the Supporting Information) or as reported in the literature.