Figure 2c:

In vitro studies. (a) Immunofluorescence image shows CC-531 cells that were incubated with anti-MG1 mAb–conjugated HNP for 24 hours and then fixed. Immunofluorescence was performed. Image on left is CC-531 cells stained with 4ʹ,6-diamidine-2-phenylindole. Middle image shows fluorescent anti-MG1 mAb-conjugated HNP. Image on the right is coregistered and shows selective binding of anti-MG1 mAb-conjugated HNP to CC-531 (10 μm). (b) Transmission electron microscopic image shows CC-531 cells that were incubated with HNP for 24 hours and then fixed (10 μm). Images show (b) cell beginning to undergo endocytosis in cluster of anti-MG1 mAb-conjugated HNPs (arrow, 500 nm) and (c) endocytoma-containing anti-MG1 mAb-conjugated HNP (arrow, 1 μm).