Figure 4b:

Histologic evaluation. (a) Representative photomicrograph shows hematoxylin-eosin–stained section demonstrating large colorectal liver metastasis with densely packed tumor cells (magnification, ×20). (b) Prussian blue staining (iron stains blue) shows deposition of anti-MG1–conjugated HNP around tumor. Image in inset shows Prussian blue staining at higher magnification (×20) in tumor. Hematoxylin-eosin slides were analyzed and total tumor area (densely packed areas of liver) and area of necrosis (dashed lines) were determined for control group, (c) HNP group, and (d) mAb-conjugated HNP group (magnification, ×20). (e) Hematoxylin-eosin slides were evaluated to determine percentage of necrosis, and control, HNP (NP), and mAb-conjugated anti-MG1 HNPs were compared (P = .043).