Fig. 1. Isolation and characterization of CcmK4:eGFP β-carboxysomes from Syn7942. (A) Step sucrose gradient separation of CcmK4:eGFP β-carboxysomes. (B) Fluorescence detection of β-carboxysomes fused with GFP in different sucrose fractions. (C) SDS-PAGE of individual fractions from the β-carboxysome purification, showing the polypeptide composition of isolated β-carboxysomes. The presence of Rubisco was verified by immunoblot analysis (Fig. S1†). Determination of β-carboxysome proteins was confirmed by proteomic analysis (Table S1†). (D) Rubisco activities of each sucrose fractions determined by 14C radiometric assay.
