Table 1. Thermal melting (T_m) data for duplexes containing a single triazole linkage.

ON code	ON sequence (5′–3′)	DNA target		RNA target
		T m a	ΔTm b	T m a	ΔTm b
ON1	CGACGMeCTTGCAGC	64.2		62.8
ON2	CGACGMeCtTLTGCAGC	58.2	–6.0	62.0	–0.8
ON3	CGACGMeCtTTG CAGC	55.3	–8.9	56.6	–6.2
ON4	CGACGMeCTLTGCAGC	67.5	+3.3	68.9	+6.1
ON5	CGACGMeCLtTTGCAGC	52.7	–11.5	55.5	–7.2
ON6	CGACGMeCLtTLTGCAGC	58.4	–5.8	62.9	+0.1

^aMelting temperatures (T_m) were obtained from the maxima of the first derivatives of the melting curves (A₂₆₀vs. temperature) recorded in a buffer containing 10 mM phosphate and 200 mM NaCl at pH 7.0 using 3.0 μM concentrations of each strand.

^bΔT_m = change in T_m for a modified duplex relative to the unmodified duplex. T^L is LNA thymidine, ^MeC is 5-methylcytosine and t is a triazole linkage (Fig. 1a). DNA target: 5′-dGCT GCA AGC GTC G. RNA target: 5′-rGCU GCA AGC GUC G.