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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Biotechnol J. 2017 Apr 20;12(5):10.1002/biot.201600696. doi: 10.1002/biot.201600696

Figure 1.

Figure 1

Schematic of yeast surface display strategies. (A) Conventional vectors, pTMY (N-terminal Aga2p fusion) and pCT (C-terminal Aga2p fusion). (B) pCL, a vector that enables co-expression of two proteins on the N- and C-terminus of the Aga2p subunit. (C) The expression cassette of pCL in which one protein is inserted between the synthetic prepro signal peptide and Aga2p and the other protein is inserted downstream of Aga2p. Various epitope tags (HA, c-Myc and FLAG) are included to validate and compare protein expression across formats.