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. Author manuscript; available in PMC: 2017 Nov 30.
Published in final edited form as: Cell. 2016 May 26;165(7):1776–1788. doi: 10.1016/j.cell.2016.05.010

Figure 1. Behavioral Cohort-Scale Brain-wide Activity Mapping.

Figure 1

(A) Schematic of ArcTRAP labeling and the enhanced cohort-scale CLARITY pipeline for rapid whole-brain clearing and imaging. CreER expression is driven by the activity-dependent Arc promoter to mediate 4TM-dependent recombination that permanently labels the active neurons with tdTomato.

(B) Representative confocal images from 40-μm sections showing TRAP labeling in BLA (yellow circle). Scale bar, 400 μm.

(C) 3D rendering of a CLARITY-processed whole-mouse brain (ArcTRAP) imaged by light-sheet microscopy. Scale bar, 500 μm.

(D) Top: single field of view (FOV) images at the indicated imaging depths. Bottom: zoomed-in images from the yellow-boxed regions in the top row showing cellular resolution. Scale bar, 100 μm.

See also Figure S1.