Table 1. Primers used for studying reference gene expression in A. gifuensis by qRT-PCR.
| Gene | Primer sequence (5’-3’) | Product length (bp) | Primer efficiency (%E) | Regression coefficient (R2) |
|---|---|---|---|---|
| DIMT |
F:AAGCAGCAATAAGACCATC R:TCTAATTCAGCAACCATACG |
133 | 91.56 | 0.9950 |
| ACTB |
F:GCTGTTGTGGTGAATGAG R:CAATCTATGAAGGTTATGCTCTT |
121 | 93.04 | 0.9943 |
| RPL3 |
F:TGATTGATGTTATTGGTGTTAC R:GAATGATACTCTGCTTGGAT |
146 | 92.63 | 0.9941 |
| PPI |
F:CAAGACGTGAACCAGAAGA R:TGCTGTATGTATTATTGCTGTATC |
143 | 93.61 | 0.9803 |
| TBP |
F:CAATAATGCCGCTTCATC R:ACTTCATCCAGGTGTTAC |
184 | 93.04 | 0.9711 |
| RPII3 |
F:CTTGTGAGGCTCTTGATTC R:GAGGCGAGGTAAAGTGTA |
75 | 88.96 | 0.9952 |
| 18SrRNA |
F:CTATGAGTCTGGTAATTGGAAT R:GCAACAACTTAAATATACGCTAT |
123 | 95.76 | 0.9880 |
| RPS18 |
F:GGTTAGCGATGATAGTTACAAT R:CAACATAGATGGCAACAGAA |
167 | 92.63 | 0.9921 |
| AK |
F:CTTGTCTGTCTTGCTGAA R:CGATTCTGGTGTTGGTATT |
112 | 96.2 | 0.9893 |
| EF1A |
F:AACAACCAACACCAACAC R:TAGGCATACCACGACTTC |
153 | 90.37 | 0.9773 |
| RPL27 |
F:CTGATACCAATGTCCACAA R:CCACCACAGAATCAACTT |
134 | 92.62 | 0.9911 |
| RPL29 |
F:GGAATCAAGAAGCCAACAC R:AATCTCTGGTTACGAAGGAAT |
77 | 92.21 | 0.9896 |