Table 2. Stability of reference gene expression under four experimental conditions.
| Experimentconditions | Reference gene | geNorm | NormFider | BestKeeper | ΔCt | ||||
|---|---|---|---|---|---|---|---|---|---|
| Stability | Rank | Stability | Rank | Stability | Rank | Stability | Rank | ||
| Developmental stage | DIMT | 0.365 | 3 | 0.96 | 7 | 0.611 | 1 | 1.21 | 7 |
| AK | 0.706 | 5 | 0.58 | 3 | 0.912 | 7 | 1.096 | 5 | |
| PPI | 0.323 | 2 | 0.542 | 2 | 0.741 | 4 | 1.007 | 2 | |
| RPII3 | 0.257 | 1 | 0.723 | 6 | 0.621 | 2 | 1.077 | 4 | |
| TBP | 1.07 | 9 | 1.167 | 10 | 1.685 | 11 | 1.413 | 10 | |
| ACTB | 0.583 | 4 | 0.345 | 1 | 1.105 | 8 | 1.001 | 1 | |
| 18SrRNA | 1.248 | 11 | 1.567 | 12 | 1.269 | 9 | 1.775 | 12 | |
| RPS18 | 0.991 | 8 | 1.094 | 9 | 1.561 | 10 | 1.366 | 8 | |
| RPL13 | 0.257 | 1 | 0.665 | 5 | 0.694 | 3 | 1.033 | 3 | |
| RPL27 | 0.859 | 7 | 1.072 | 8 | 0.82 | 6 | 1.375 | 9 | |
| RPL29 | 1.143 | 10 | 1.238 | 11 | 1.707 | 12 | 1.502 | 11 | |
| EF1A | 0.772 | 6 | 0.621 | 4 | 0.764 | 5 | 1.12 | 6 | |
| Sex | DIMT | 1.136 | 10 | 1.474 | 11 | 2.238 | 11 | 1.57 | 11 |
| AK | 0.369 | 3 | 0.428 | 4 | 0.622 | 4 | 0.96 | 4 | |
| PPI | 0.4 | 4 | 0.255 | 2 | 0.96 | 8 | 0.95 | 3 | |
| RPII3 | 0.865 | 8 | 0.569 | 5 | 1.502 | 9 | 1.1 | 6 | |
| TBP | 1.224 | 11 | 1.587 | 12 | 2.327 | 12 | 1.66 | 12 | |
| ACTB | 0.286 | 1 | 0.349 | 3 | 0.708 | 6 | 0.94 | 2 | |
| 18SrRNA | 0.662 | 6 | 1.36 | 9 | 0.289 | 1 | 1.51 | 9 | |
| RPS18 | 0.286 | 1 | 0.148 | 1 | 0.822 | 7 | 0.89 | 1 | |
| RPL13 | 0.34 | 2 | 0.588 | 6 | 0.542 | 2 | 1 | 5 | |
| RPL27 | 0.769 | 7 | 1.371 | 10 | 0.623 | 5 | 1.54 | 10 | |
| L29 | 1.007 | 9 | 1.165 | 8 | 1.923 | 10 | 1.4 | 8 | |
| EF1A | 0.52 | 5 | 0.795 | 7 | 0.62 | 3 | 1.16 | 7 | |
| Tissue | DIMT | 1.032 | 5 | 0.721 | 3 | 1.299 | 5 | 1.502 | 3 |
| AK | 1.369 | 8 | 1.253 | 8 | 0.689 | 2 | 1.803 | 8 | |
| PPI | 0.985 | 4 | 0.824 | 4 | 1.543 | 7 | 1.535 | 4 | |
| RPII3 | 1.157 | 6 | 0.616 | 2 | 0.709 | 3 | 1.48 | 2 | |
| TBP | 0.588 | 2 | 1.17 | 7 | 1.586 | 8 | 1.712 | 7 | |
| ACTB | 1.286 | 7 | 1.105 | 6 | 0.438 | 1 | 1.683 | 6 | |
| 18SrRNA | 1.428 | 9 | 1.321 | 9 | 1.151 | 4 | 1.838 | 10 | |
| RPS18 | 0.416 | 1 | 1.082 | 5 | 1.809 | 10 | 1.625 | 5 | |
| RPL13 | 0.851 | 3 | 0.37 | 1 | 1.416 | 6 | 1.372 | 1 | |
| RPL27 | 1.821 | 11 | 2.556 | 12 | 1.978 | 12 | 2.738 | 12 | |
| L29 | 0.416 | 1 | 1.37 | 10 | 1.953 | 11 | 1.804 | 9 | |
| EF1A | 1.632 | 10 | 2.529 | 11 | 1.757 | 9 | 2.735 | 11 | |
| Diet | DIMT | 0.221 | 2 | 0.219 | 3 | 0.479 | 5 | 0.653 | 3 |
| AK | 0.35 | 4 | 0.334 | 4 | 0.554 | 7 | 0.706 | 5 | |
| PPI | 0.41 | 5 | 0.499 | 7 | 0.518 | 6 | 0.785 | 7 | |
| RPII3 | 0.519 | 7 | 0.46 | 6 | 0.725 | 8 | 0.771 | 6 | |
| TBP | 0.746 | 10 | 1.303 | 11 | 1.168 | 11 | 1.398 | 11 | |
| ACTB | 0.27 | 3 | 0.353 | 5 | 0.399 | 1 | 0.698 | 4 | |
| 18SrRNA | 0.858 | 11 | 1.326 | 12 | 1.201 | 12 | 1.418 | 12 | |
| RPS18 | 0.619 | 9 | 0.772 | 10 | 0.861 | 10 | 0.968 | 10 | |
| RPL13 | 0.143 | 1 | 0.071 | 1 | 0.444 | 3 | 0.61 | 1 | |
| RPL27 | 0.143 | 1 | 0.083 | 2 | 0.422 | 2 | 0.61 | 2 | |
| L29 | 0.557 | 8 | 0.51 | 8 | 0.8 | 9 | 0.817 | 8 | |
| EF1A | 0.464 | 6 | 0.631 | 9 | 0.466 | 4 | 0.86 | 9 | |