Table 3. Pleural fluid characteristics for individual experiments.
| Experiment | S. pneumoniae in pleural fluid | S. pneumoniae in different medium | Other bacterial pathogens | S. pneumoniae D39 mutants* including ΔpsaA | S. pneumoniae D39 nutrient transporters** | S. pneumoniae ΔpsaA + manganese (Mn) |
|---|---|---|---|---|---|---|
| Pleural Fluid Samples, n | 11 | 6 | 7 | 8 | 8 | 16 |
| Pleural Fluid ID † | 1–11 | 12–17 | 47–53 | 18–25 | 26–33 | 31–46 |
| Number of bacterial strains used | 25 S. pneumoniae | 3 S. pneumoniae | 14 non-pneumococci 2 S. pneumoniae |
1 D39 wild-type 6 D39 mutants* |
1 D39 wild-type 5 D39 mutants** |
1 D39 wild 1 D39 ΔpsaA |
| Number of aliquots inoculated | 275 pleural + cells 275 pleural—cells |
18 pleural + cells 27 other media |
112 pleural + cells | 56 pleural + cells | 48 pleural + cells | 32 pleural + cells 32 pleural + cells +Mn |
|
Sex, male (%) Age, mean ±SD |
7 (63.6%) 63 years (SD±)] |
3 (50%) 70 years (SD±14) |
4 (57.1%) 74 years (SD±4) |
3 (37.5%) 70 years (SD±10) |
7 (87.5%) 69 years (SD±8) |
13 (81.2%) 69 years (SD±8) |
| pH, mean (SD) | 7.33 (SD±0.11) | 7.28 (SD±0.11) | 7.37 (SD±0.11) | 7.34 (SD±0.15) | 7.33 (SD±0.12) | 7.32 (SD±0.12) |
| LDH, U/L median (IQR) | 303 (116–4350) | 251 (218–397) | 245 (82.5–392) | 319 (189–752) | 391 (200–574) | 379 (200–574) |
| Protein, g/L median (IQR) | 39 (36–44) | 31 (23–39) | 31 (24–35) | 34 (26–40) | 39 (17–41) | 39 (17–40) |
| Glucose, mmol/L, mean (SD) | 4.09 (SD±1.9) | 4.9 (SD±1.8) | 6.3 (SD±2.3) | 4.9 (SD±2.4) | 8.4 (SD±8.4) | 6.8 (SD±6.0) |
† Individual pleural fluid characteristics are presented in S1 Table.
*Δply, ΔlytA, ΔpspA, ΔpsaA, ΔluxS, ΔcbpD,
** ΔpitA, ΔpiuB/piaA, ΔadcAI, ΔadcAII/adcAI, ΔlivH