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. 2017 Nov 30;12(11):e0188981. doi: 10.1371/journal.pone.0188981

Fig 5. Absence of changes in expression of fibrotic factors in Akr1b3 null I/R mice hearts after 28 days of recovery.

Fig 5

Mice were subjected to LAD and studies were done 28 days post infarction recovery. (A) Echocardiographic measurements made in WT and Akr1b3 null mice. Functional measurements, fractional shortening (FS) and fractional area change (FAC) are expressed as %, with representative image, N = 10 mice/group. (B) qRT-PCR on collagens (Col1a1, Col1a2, Col3a1) and MMPs (Mmp2 and Mmp9) from heart tissue, N = 5 mice/group. (C) Western blot from heart lysates for detection of β-catenin and TGFB2 normalized to Beta-Actin, N = 4 mice/group. (D) Total collagen levels in hearts from mice subjected to LAD post 48 h and 28 days of recovery, N = 4 mice/group. Error bars represent mean ± SEM. * p<0.05, unless otherwise noted.