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. 2017 Nov 30;12(11):e0188072. doi: 10.1371/journal.pone.0188072

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© 2017 Fernandez-Pernas et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — A) Selective enrichment of CD105⁺ expressing cells. Fluorescence-activated cell sorting (FACS) analysis of the subpopulations of CD105⁺ pre-sorted (on the top) and post-sorted (on the bottom) from synovial membrane. 105 after was subpopulation of CD105⁺ post-sorted (on the left bottom) and 105- after was subpopulation of CD105^- post-sorted (on the right bottom). B) Characterization by flow cytometry analysis of the CD105⁺-MSC population using MSCs markers, CD44, CD105, CD90 and haematopoietic markers CD45 and CD34. C) Flow cytometry of subpopulation of CD105⁺-MSC labelled with oxacarbocyanine (DiO) and octadecyl (C18) indocarbocyanine (DiI) respectively. D) Characterization of GFP-CD105⁺-MSCs used to validate previous data obtained using DiO- CD105⁺-MSCs and DiI-CD105⁺-MSCs in animal model. Fluorescence-activated cell sorting (FACS) analysis of the MSCs populations (on the left), GFP-MSCs (on the middle) and post-sorted for CD105⁺ (on the right) from synovial membrane. Representative image of GFP-CD105⁺-MSCs in a plate before injection, bar represents 20 μm.