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. 2017 Nov 30;12(11):e0188072. doi: 10.1371/journal.pone.0188072

Fig 3. Immunofluorescence study from animal organs.

Fig 3

A) Immunofluorescence analyses of spleen, ganglion, kidney, liver and lung from animals scarified after MSCs injections. All pictures have the same magnification, bar represents 20 μm except spleen on the right which bar represents 10 μm. B) Immunofluorescence analysis from healthy right knee from animals euthanized after MSCs injections. All pictures have the same magnification, bar represents 20 μm. C) Immunofluorescence analysis of sections of patella femoral groove, crossed ligaments, synovial membrane, internal meniscus, external meniscus and knee muscle (vastus medialis) of the animals injected IA with DiO-CD105+-MSCs and IV with DiI-CD105+-MSCs. All the sections are from the left knee which was injured and it was taken as OA (magnification 20x). The arrow points DiO-CD105+-MSCs and DiI-CD105+-MSCs very close between them. D) Histogram showed percentage of DiO and DiI positives signal normalized with DAPI signal from animals injected IA. AnalySIS Image Processing was used.