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. 2017 Nov 30;12(11):e0188778. doi: 10.1371/journal.pone.0188778

Fig 9. Actin-pl-clusters were found in intact HeLa cells as well as in the NRK cells extensively used in this study: After the addition of 200 nM latrunculin A, actin-pl-clusters disappeared in 1~2 min in both HeLa and NRK cells, but in HeLa cells, actin clusters much larger than the actin-pl-clusters started appearing between 600 and 750 s after latrunculin addition.

Fig 9

The figure shows representative snapshots from image sequences of HeLa cells (Left) and NRK cells (Right) transfected with Lifeact-mGFP and observed by SRM (an Olympus SD-OSR system). After the snapshots at time = 0 (0 s), the cells were treated with 200 nM latrunculin A and time-lapse observations were performed for about 1,000 s.