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. 2017 Nov 30;12(11):e0188778. doi: 10.1371/journal.pone.0188778

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© 2017 Shirai et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 10 — Two-color TIRFM observations of (Top row) Lifeact-mGFP (green) and Halo-N-WASP labeled with TMR (red) (see S4 Movie), (Second row) Lifeact-Halo labeled with TMR (green) and EGFP-cortactin (red), (Third row) Lifeact-mGFP (green) and Halo-Tks4 labeled with TMR (red) (see S5 Movie), (Fourth row) Lifeact-mGFP (green) and Halo-Tks5 labeled with TMR (red) (see S6 Movie) and (Bottom row) Lifeact-mGFP (green) and Halo-paxillin labeled with TMR. The observation conditions and the analyses were the same as in Fig 8. Statistically significant colocalizations with actin-pl-clusters were found for N-WASP, cortactin, Tks4, and Tks5.