Fig 13. Actin-pl-clusters exhibit dynamic morphological changes and podosome-related proteins are recruited to actin-pl-clusters.

Actin-pl-clusters underwent dynamic motion and morphological changes on or near the PM. These include (a) elongation, (b) splitting, (c) directed movement along actin meshes, (d) merging, (e) shrinking, and (f) spreading (often forming a fork-like morphology). N-WASP, Tks4, and Tks5 molecules were either directly recruited to the actin-pl-cluster from the cytoplasm (60–70% of molecules) or first arrived at the PM outside the cluster and were then recruited to the cluster by lateral diffusion on the PM cytoplasmic surface. They left the actin-pl-clusters either by direct dissociation into the cytoplasm (approximately 70% of molecules) or by lateral diffusion on the PM cytoplasmic surface.