Table 1. The percentage of TALL events that Lifeact-TM molecules exhibited on actin-pl-clusters, stress fibers, and the cortical actin meshwork (the PM regions on which these structures are projected), and the percentage of at least one TALL event once Lifeact-TM entered the PM region on the three actin structures (the projected PM regions).
| PM region | %TALLs occurred (Mean ± SE) |
%TALLs once Lifeact-TM entered the PM region on the actin structure (Mean ± SE) |
Total # of TALLs |
Total # of structures |
Total # of cells examined |
|---|---|---|---|---|---|
| Actin-pl-clusters | 12.8 ± 2.2 | 71.5 ± 4.3 | 116 | 94 | 5 |
| Stress fibers | 20.8 ± 6.9 | 89.4 ± 4.8 | 196 | 46 | 4* |
| Cortical meshwork | 66.4 ± 6.8 | N.A.† | 628 | N.A.* | 5 |
*One of the five cells inspected did not show the presence of stress fibers, and therefore, the number of cells examined is one less than those of the other structures.
†Not applicable because the regions covered by the cortical actin meshwork are often well connected and represent very high percentages of the PM area, making the evaluation meaningless (~100% for almost all of the cells).