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. 2017 Nov 30;12(11):e0188963. doi: 10.1371/journal.pone.0188963

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© 2017 Mathavan et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — Western blots of EC1-3-myc (A) and non-adhesive EC1-3-myc (B; naEC1-3-myc) co-immunoprecipitations (coIPs). Receptors were immunoprecipitated using anti-HA or anti-flag antibodies. Both EC1-3 constructs co-IPed with all receptors: HA-tagged EGFR/ErbB1, HA-tagged ErbB3 and flag-tagged ErbB2, ErbB4, PDGFRα and FGFR1. (C) Western blot of EC1-3-myc coIP with cytosolic proteins ARID3a-flag and importinβ1-flag. To test if EC1-3 could interact non-specifically with proteins following extraction we tested Flag-tagged constructs overexpressed in Hek293T cells. EC1-3-myc did not associate with ARID3a-flag, nor importinβ1-flag. ErbB2-flag was used as a positive control. Results are representative of three independent experiments.