Skip to main content
. 2017 Nov 30;12(11):e0188963. doi: 10.1371/journal.pone.0188963

Fig 5. ErbB2-ErbB3 heterodimers bind PI3K p85 in the presence of recombinant EC1-3 (bacEC).

Fig 5

(A) Quantitative real-time PCR analysis of ErbB receptors from dissected CNC and stage 16 embryos. The relative gene expression in the CNC is compared to that of whole embryos at the same stage. ErbB2, 3, and 4 are enriched in the CNC compared to whole embryos. Slug was used as a positive control for CNC enrichment. GAPDH was used for normalization. (B) Western blot of co-immunoprecipitation experiments with ErbB3-HA and p85 in the presence or absence of bacEC and ErbB2-flag. Hek293T cells were transfected with ErbB3-HA alone or together with ErbB2-flag, serum starved, and treated with 10 ng/mL bacEC for 5 minutes before being lysed for co-immunoprecipitation experiments. ErbB3-HA modestly binds p85 when transfected alone. Co-transfection of ErbB3-HA with ErbB2-flag noticeably increases this interaction. Association of p85 with ErbB3-HA is further elevated in the presence of bacEC. (C) Co-precipitation of p85 with ErbB3-HA and ErbB2-flag significantly increases (p = 0.03) in the presence of bacEC. Immunoprecipitated ErbB3-HA was used for normalization. Results are representative of three independent experiments.