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. 2017 Nov 30;12(11):e0188871. doi: 10.1371/journal.pone.0188871

Fig 4. ERRα is stabilized by LSD1 independently of the latter’s enzymatic activity.

Fig 4

a. Expression of ERRα protein in HeLa cells transfection with the indicated amount of myc-tagged LSD1. b. Steady state level of endogenous ERRα protein in HeLa cells transfected with myc-tagged wild type or catalytic mutant (K661A) LSD1, after CHX treatment (100 mg/ml) for the indicated time. Western blots were probed with the indicated antibodies and quantification of ERRα levels (relative to hsp90 or actin) is displayed. c. HeLa cells were transfected with flag-ERRα and together with myc-tagged LSD1 derivatives as indicated above. Co-immunoprecipitations were performed using flag antibody. flag-ERRα and myc-LSD1 were detected using the corresponding antibodies. d. Co-immunoprecipitation of endogenous proteins with anti-ERRα antibody (or rabbit IgG as control) from HeLa cells treated with pargyline or vehicle (-) in the presence of MG132.