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12% SDS-PAGE (left) and casein zymogram (right) of ammonium sulphate precipitate of culture supernatant obtained from Bacillus sp. SKS1 showing three protease bands of approximately (A) 120 kDa, (B) 66 kDa and (C) 35 kDa. (Lane 1: unstained marker, lane 2: ammonium sulphate precipitated protease, lane 3: ammonium sulphate precipitated protease heated at 60°C for an hour).
