Table 3. Buffers used for determining pH effect on enzyme activity.

Buffers (0.1 M)	pH
Potassium phosphate buffer	6
Potassium phosphate buffer	7
Potassium phosphate buffer	8
Sodium carbonate–bicarbonate buffer	9
Sodium carbonate–bicarbonate buffer	10
Sodium bicarbonate–sodium hydroxide buffer	11
Potassium chloride–sodium hydroxide buffer	12

Various buffers having pH ranging from 6 to 12 were used to study effect of pH on protease activity.