Table 8. Purification of enzyme produced by Bacillus infantis SKS1.
Purification step | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Yield (%) | Purification fold |
---|---|---|---|---|---|
Culture supernatant | 375 | 47.27 | 7.93 | 100 | 1 |
Ammonium sulphate precipitation | 328.5 | 15.44 | 21.27 | 87.6 | 2.68 |
Partial purification of enzyme produced by Bacillus infantis SKS1 was achieved using ammonium sulphate precipitation method.