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. 2017 Nov 30;12(11):e0188506. doi: 10.1371/journal.pone.0188506

Table 1. PCR primers.

Gene Reference sequence number Sequence Product length
Hypoxanthine guanine phosphoribosyltransferase. HPRT NM012583 Forward 5´AAGCTTGCTGGTGAAAAGG3´
Reverse 3´CAAAGCCTAAAAGACAGCGG5´
192 pb
Interferon gamma. IFNɣ NM138880
Forward 5’AACCAGGCCATCAGCAACA3’
Reverse 3’TCTGTGGGTTGTTCACCTCG5’
128 pb
Tumor necrosis factor alpha. TNF α NM012675
Forward 5’CTCTTCTGTCTACTGAACT3’
Reverse 3’GAGAAGATGATCTGAGTGTG5’
115 pb
Interleukin 4. IL4 NM201270 Forward 5’GGCTTCCAGGGTGCTTCGC3’
Reverse 3’GTGGACTCATTCACGGTGCA5’
150 pb
Interleukin 10. IL10
X60675 Forward 5’GGGGTGACAATAACTGCAT3’
Reverse 3’GGGGCATCACTTCTACCAT5’
216 pb
Transforming growth factor, beta 3. TGFb3 NM013174 Forward 5´CCCAACCCCAGCTCCAAGC3´
Reverse 3´AGCCACTCTGCGGTGCCTCG5´
132 pb
Forkhead box P3. Foxp3 NM110825 Forward 5´CTGCCTGTGCTTAGGAGAC3´
Reverse 3´CATCACTGGCTTTCGCGTA5´
156 pb