Table 1. PCR primers.
Gene | Reference sequence number | Sequence | Product length |
---|---|---|---|
Hypoxanthine guanine phosphoribosyltransferase. HPRT | NM012583 | Forward 5´AAGCTTGCTGGTGAAAAGG3´ Reverse 3´CAAAGCCTAAAAGACAGCGG5´ |
192 pb |
Interferon gamma. IFNɣ | NM138880 |
Forward 5’AACCAGGCCATCAGCAACA3’ Reverse 3’TCTGTGGGTTGTTCACCTCG5’ |
128 pb |
Tumor necrosis factor alpha. TNF α | NM012675 |
Forward 5’CTCTTCTGTCTACTGAACT3’ Reverse 3’GAGAAGATGATCTGAGTGTG5’ |
115 pb |
Interleukin 4. IL4 | NM201270 | Forward 5’GGCTTCCAGGGTGCTTCGC3’ Reverse 3’GTGGACTCATTCACGGTGCA5’ |
150 pb |
Interleukin 10. IL10 |
X60675 | Forward 5’GGGGTGACAATAACTGCAT3’ Reverse 3’GGGGCATCACTTCTACCAT5’ |
216 pb |
Transforming growth factor, beta 3. TGFb3 | NM013174 | Forward 5´CCCAACCCCAGCTCCAAGC3´ Reverse 3´AGCCACTCTGCGGTGCCTCG5´ |
132 pb |
Forkhead box P3. Foxp3 | NM110825 | Forward 5´CTGCCTGTGCTTAGGAGAC3´ Reverse 3´CATCACTGGCTTTCGCGTA5´ |
156 pb |