Fig 5.

DHCA induced ROS generation and arrested cells in S- and G2/M phase of cell cycle: (A) HDAC inhibitor DHCA increased ROS levels in a dose and time dependent manner to induce cell death in HCT-116 cell lines. Further, in presence of an external ROS generator like H₂O₂, the production of ROS was much higher compared to H₂O₂ and DHCA alone, indicating that these molecules cause cell death through ROS induction. The numbers above the graph indicate percentage viable cells. (B) DHCA also enhanced the ROS production in HCT-15 cell lines similar to HCT-116 cells. The effect of compounds on ROS production was found to be dose dependent. (C) Analysis of HCT-116 cells treated with DHCA showed induction of apoptotic sub-G0-G1 population and arrest of cells in S- and G2/M phase. (D) DHCA treatment arrested HCT-15 cells in S phase in a dose dependent fashion.